90i fluorescent microscope Search Results


human imr90 fetal lung fibroblasts  (ATCC)
99
ATCC human imr90 fetal lung fibroblasts
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Human Imr90 Fetal Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human imr90 fetal lung fibroblasts/product/ATCC
Average 99 stars, based on 1 article reviews
human imr90 fetal lung fibroblasts - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
eclipse 90i widefield fluorescent microscope  (Nikon)
99
Nikon eclipse 90i widefield fluorescent microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Eclipse 90i Widefield Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse 90i widefield fluorescent microscope/product/Nikon
Average 99 stars, based on 1 article reviews
eclipse 90i widefield fluorescent microscope - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
eclipse 90i fluorescence microscope  (Nikon)
96
Nikon eclipse 90i fluorescence microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Eclipse 90i Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse 90i fluorescence microscope/product/Nikon
Average 96 stars, based on 1 article reviews
eclipse 90i fluorescence microscope - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
fluorescence microscope  (Nikon)
96
Nikon fluorescence microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence microscope/product/Nikon
Average 96 stars, based on 1 article reviews
fluorescence microscope - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
camera hamamatsu orca-er  (Hamamatsu)
90
Hamamatsu camera hamamatsu orca-er
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Camera Hamamatsu Orca Er, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/camera hamamatsu orca-er/product/Hamamatsu
Average 90 stars, based on 1 article reviews
camera hamamatsu orca-er - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
fv1000 confocal microscope  (Evident Corporation)
90
Evident Corporation fv1000 confocal microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Fv1000 Confocal Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fv1000 confocal microscope/product/Evident Corporation
Average 90 stars, based on 1 article reviews
fv1000 confocal microscope - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
inverted nikon fluorescence microscope  (Nikon)
99
Nikon inverted nikon fluorescence microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Inverted Nikon Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted nikon fluorescence microscope/product/Nikon
Average 99 stars, based on 1 article reviews
inverted nikon fluorescence microscope - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
90i c1 fluorescent microscope  (Nikon)
99
Nikon 90i c1 fluorescent microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
90i C1 Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/90i c1 fluorescent microscope/product/Nikon
Average 99 stars, based on 1 article reviews
90i c1 fluorescent microscope - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
epi fluorescence microscope  (Nikon)
96
Nikon epi fluorescence microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Epi Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/epi fluorescence microscope/product/Nikon
Average 96 stars, based on 1 article reviews
epi fluorescence microscope - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
hamamatsu orca-er camera  (Hamamatsu)
90
Hamamatsu hamamatsu orca-er camera
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Hamamatsu Orca Er Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hamamatsu orca-er camera/product/Hamamatsu
Average 90 stars, based on 1 article reviews
hamamatsu orca-er camera - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
lsm710 multiphoton confocal laser scanning microscope  (Carl Zeiss)
90
Carl Zeiss lsm710 multiphoton confocal laser scanning microscope
A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in <t>IMR90</t> eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.
Lsm710 Multiphoton Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lsm710 multiphoton confocal laser scanning microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
lsm710 multiphoton confocal laser scanning microscope - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in IMR90 eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.

Journal: bioRxiv

Article Title: Interplay between PML NBs and HIRA for H3.3 deposition on transcriptionally active interferon-stimulated genes

doi: 10.1101/2021.11.30.470516

Figure Lengend Snippet: A. (left) Fluorescence microscopy visualization of HIRA (green) and PML (red) in BJ cells treated with a control siRNA targeting luciferase (siLuc) or with an siRNA against DAXX (siDAXX) for 48h. Ruxolitinib (Ruxo) was added at 2μM in the last 24h. Cell nuclei are visualized by DAPI staining (grey). Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs. p-values (Student t-test): *<0,05; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). B. Histogram shows quantitative analysis of PML protein levels from western blot analysis presented in Sup. Figure 3C. p-values (Student t-test): ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). C. (left) Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin (Dox) and IFNβ for 24h. Arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment. Scale bars represent 10μm. (right) Histogram shows quantitative analysis of cells with HIRA localization at PML NBs in BJ eH3.3i treated with Dox and IFNβ as indicated for 24h. p-values (Student t-test): *<0,05; **<0,01; ns: non significant. Numbers represent the mean of 3 independent experiments (±SD). D. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in BJ eH3.3i cells treated with doxycyclin and with or without IFNβ for 24h. High exposure indicates a lane where H3.3-HA signal was specifically increased in order to show H3.3-HA localization in PML NBs without saturating the signal in cells with higher expression. Scale bars represent 10μm. E. Quantification of nuclear H3.3-HA intensity levels in BJ eH3.3i cells treated as in C. Mean H3.3-HA intensity levels were calculated on a pool of n=121 nuclei from 3 independent experiments (total). Nuclei were then separated on basis of accumulation of HIRA in PML NBs (nuclei with HIRA in PML NBs, n=58) or without it (nuclei with no HIRA in PML NBs, n=63) and mean H3.3-HA intensity was plotted for each category. Bars represent median with interquartile range. p-values (Mann-Whitney u-test): ****<0,0001. F. Fluorescence microscopy visualization of HIRA (green) and H3.3-HA (red) in IMR90 eH3.3i cells treated as in C. Scale bars represent 10μm. Arrows indicate nuclei showing accumulation of HIRA in PML NBs together with H3.3-HA, while arrowheads indicate nuclei with high levels of nucleoplasmic H3.3-HA preventing HIRA accumulation in PML NBs despite IFNβ treatment.

Article Snippet: Human BJ primary foreskin fibroblasts (ATCC, CRL-2522), Human IMR90 fetal lung fibroblasts (ATCC, CCL-186), human HEK 293T embryonic kidney cells (Intercell, AG) and mouse MEFs embryonic fibroblasts Pml -/- (from Dr. Lallemand-Breitenbach) were cultivated in DMEM medium (Sigma-Aldrich, D6429) containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich, P4458), at 37°C under 5% CO2 and humid atmosphere.

Techniques: Fluorescence, Microscopy, Control, Luciferase, Staining, Western Blot, Expressing, MANN-WHITNEY